A method is described which employs differential centrifugation and sucrose density gradient centrifugation to isolate a membrane fraction from human lymphocytes. Membrane preparations from long-term human cultured B- and T-lymphoid lines, peripheral blood lymphocytes, tonsillar lymphocytes, and thymocytes were analyzed on 0.5% sodium dodecyl sulfate-7.5% polyacrylamide gels stained for protein and carbohydrate. The most important finding was a major glycoprotein of approximately 30,000 daltons associated with the membrane preparations from B lymphocytes. T-lymphocyte preparations did not contain readily detectable amounts of this membrane-associated component. The T-cell lymphoid line MOLT-4 was unique in that it had a narrow protein band at approximately 30,000 daltons which did not contain carbohydrate.
Article|
December 01 1975
A glycoprotein associated with the membrane fraction of human B but not T lymphocytes.
S Fujita
S D Litwin
N Hartman
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1975) 142 (6): 1416–1424.
Citation
S Fujita, S D Litwin, N Hartman; A glycoprotein associated with the membrane fraction of human B but not T lymphocytes.. J Exp Med 1 December 1975; 142 (6): 1416–1424. doi: https://doi.org/10.1084/jem.142.6.1416
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