The following observations indicate that cultured human WI-38 fibroblasts synthesize and secrete alpha2-macroglobulin into serum-free medium: (a) after incubation of cultures with [35S]L-methionine, a labeled protein appeared in the medium which was precipitated by antiserum directed against alpha2-macroglobulin; (b) after incubation of cultures with [35S]L-methionine, a major band of radioactivity detected by polyacrylamide gel electrophoresis of the proteins in medium co-migrated with alpha2-macroglobulin; and (c) the amount of alpha2-macroblobulin in the medium, estimated both functionally and immunologically, increased with time in normal but not not puromycin-treated cultures.
Article| February 01 1976
Synthesis and secretion of alpha2-macroglobulin by cultured human fibroblasts.
D F Mosher
D A Wing
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1976) 143 (2): 462–467.
D F Mosher, D A Wing; Synthesis and secretion of alpha2-macroglobulin by cultured human fibroblasts.. J Exp Med 1 February 1976; 143 (2): 462–467. doi: https://doi.org/10.1084/jem.143.2.462
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