We have shown that cytotoxic T cells can be polyclonally activated by a short exposure to complexes of polyadenylic:polyuridylic acid (poly A:U). Activation is optimal at a dose of 100 microgram/ml poly A:U and occurs during a 2 h incubation period in the absence of antigen. Killing of allogeneic, but not syngeneic, target cells can be observed after 12 h in culture and peaks after 21-24 h in the absence of any nonspecific binding ligand. The observed cytotoxicity is mediated by T lymphocytes and dose not require accessory macrophages or DNA synthesis for the activation or expression of effector functions. These results suggest that few requirements exist for the activation of cytotoxic T cells.
Article| September 01 1977
Cytotoxic T-cell activation by polyribonucleotides: DNA synthesis is not required.
P H Bick
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1977) 146 (3): 844–856.
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P H Bick, G Möller; Cytotoxic T-cell activation by polyribonucleotides: DNA synthesis is not required.. J Exp Med 1 September 1977; 146 (3): 844–856. doi: https://doi.org/10.1084/jem.146.3.844
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