Supernates of tetanus toxoid (TT) antigen-stimulated human T cells were studied for the presence of an antigen-specific T-cell helper factor (ASF). Supernates were circulated over an immunosorbent column consisting of insolubilized TT antigen. The material which bound to the column was eluted with 3 M NaCNS and was shown to contain a factor which in the presence of TT-induced specific IgG anti-TT antibody synthesis in autologous B cells without causing readily detectable proliferation. ASF activity was partially inhibited by antisera directed against the B-cell alloantigens of the ASF donor. Immunosorbent columns containing such antisera removed ASF activity. Immunosorbent columns containing antisera to human immunoglobulin heavy chain determinants did not remove ASF activity; whereas immunosorbent columns containing rabbit idiotypic antiserum directed against anti-TT antibodies completely removed ASF activity. ASF was destroyed by treatment with proteolytic enzymes; its molecular weight was estimated by Sephadex G-100 gel column chromatography to be between 25,000 and 75,000 daltons.
Article| October 01 1978
Antigen-specific helper factor in man.
F B Mudawwar
E J Yunis
R S Geha
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1978) 148 (4): 1032–1043.
F B Mudawwar, E J Yunis, R S Geha; Antigen-specific helper factor in man.. J Exp Med 1 October 1978; 148 (4): 1032–1043. doi: https://doi.org/10.1084/jem.148.4.1032
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