A sensitive, specific, and reproducible in vitro helper assay is described which is suited to limiting dilution analysis of murine B cells. 1 in about 3,000 syngeneic splenic B cells can be induced to form plaque-forming cells (PFC) to sheep erythrocytes in this system. The induction of PFC is absolutely dependent on antigen and specific helper T cells.
Article| December 01 1978
B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay.
M H Schreier
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1978) 148 (6): 1612–1619.
M H Schreier; B-cell precursors specific to sheep erythrocytes. Estimation of frequency in a specific helper assay.. J Exp Med 1 December 1978; 148 (6): 1612–1619. doi: https://doi.org/10.1084/jem.148.6.1612
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