NZB mice and their F1 hybrids produce excessive polyclonal IgM and autoantibodies of both IgM and IgG classes. CBA/N mice and CBA/N-mothered F1 males fail to make antibody to many T-independent antigens and have low levels of serum IgM; further, these mice lack a population of splenic B cells characterized by a low-to-intermediate density of surface IgM. We have studied male CBA/N, NZB, CBA/N X NZB, NZB X CBA/N, and CBA/J mice; female CBA/N X NZB mice; and males of several control crosses of NZB and CBA/N mice. We have found that the CBA/N X-linked defect of T-independent immune response is completely expressed in CBA/N X NZB mice. In marked contrast to NZB mice and to NZB mice and to NZB F1 hybrids bearing at least one normal X chromosome, the CBA/N X NZB males failed to respond to two T-independent antigens, had small numbers of splenic IgM-producing cells, barely detectable splenic IgM production, and splenic B-cell surface-Ig patterns resembling those of CBA/N mice. These data suggest that the NZB B-cell abnormality resulting in excessive IgM production occurs almost exclusively in that population of B cells affected by the CBA/N X chromome-linked defect. Preliminary studies suggest that CBA/N X chromosome retards the spontaneous development of anti-erythrocyte autoantibodies in CBA/N X NZB males. Castration, known to accelerate autoimmune disease in certain NZB F1 males, appears to have no influence on the immune functions examined in this study.
Article|
July 01 1979
CBA/N X-linked B-cell defect prevents NZB B-cell hyperactivity in F1 mice.
J D Taurog
H M Moutsopoulos
Y J Rosenberg
T M Chused
A D Steinberg
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1979) 150 (1): 31–43.
Citation
J D Taurog, H M Moutsopoulos, Y J Rosenberg, T M Chused, A D Steinberg; CBA/N X-linked B-cell defect prevents NZB B-cell hyperactivity in F1 mice.. J Exp Med 1 July 1979; 150 (1): 31–43. doi: https://doi.org/10.1084/jem.150.1.31
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