Short-term cultures of human peripheral blood monocytes were shown to synthesize the alternative pathway complement components C3, factors B (B) and D (D), and properdin, the regulatory proteins C3b inactivator (C3bINA) and beta 1H, in addition to C2, C4, and C5. B, D, properdin, C3bINA, and C2 were detected by functional assays, whereas beta 1H, C4, C3, and C5 could only be detected using immunochemical procedures. Immunoperoxidase localization studies showed that all the cells in each culture contained each component, so it is possible that all monocytes synthesize each component. It is concluded that cells of the monocyte-macrophage series form a mobile source of complement components and regulatory proteins which can be concentrated at sites of inflammation.
Article|
March 01 1980
Biosynthesis of the complement components and the regulatory proteins of the alternative complement pathway by human peripheral blood monocytes.
K Whaley
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1980) 151 (3): 501–516.
Citation
K Whaley; Biosynthesis of the complement components and the regulatory proteins of the alternative complement pathway by human peripheral blood monocytes.. J Exp Med 1 March 1980; 151 (3): 501–516. doi: https://doi.org/10.1084/jem.151.3.501
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