The Tla region located on the murine 17th chromosome controls several serologically defined cell surface antigens. These antigens, referred to as Qa-1-5 and TL, are expressed on a variety of hematopoeitic cell populations. In the present studies we have immunoprecipitated isotopically labeled Qa-2 and H-2 molecules from mitogen-stimulated B6 spleen cells. Sequential immunoprecipitation experiments have shown that the determinants recognized by alpha Qa-2, alpha H-2Kb, and alpha H-2Db alloantisera reside on separate molecular species. Comparative mapping of the arginine-labeled tryptic peptides from Qa-2, H-2Kb, and H-2Db molecules indicate that Qa-2 is structurally distinct but that there is considerable structural homology; 21-43% of the Qa-2 peptides co-chromatograph with peptides derived from H-2Db and H-2Kb, respectively. Similar levels of homology are observed when Qa-2 is compared with H-2Kk or H-2Dd. The results show that the Qa-2 alloantigen is encoded by a locus separate from the loci encoding H-2K or H-2D alloantigens, but that the Qa-2, H-2K, and H-2D alloantigens are sufficiently related at the primary structural level to indicate that they evolved from a common primordial gene.
Article|
May 01 1981
Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene.
M J Soloski
J W Uhr
L Flaherty
E S Vitetta
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1981) 153 (5): 1080–1093.
Citation
M J Soloski, J W Uhr, L Flaherty, E S Vitetta; Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene.. J Exp Med 1 May 1981; 153 (5): 1080–1093. doi: https://doi.org/10.1084/jem.153.5.1080
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