Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F1 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unnecessary for IL-2 production in our assay, or their effect may be produced by anti-idiotype. Thus, anti-idiotype may provide two or more specific T cell signals.
Article|
April 01 1982
Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones.
A J Infante
P D Infante
S Gillis
C G Fathman
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1982) 155 (4): 1100–1107.
Citation
A J Infante, P D Infante, S Gillis, C G Fathman; Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones.. J Exp Med 1 April 1982; 155 (4): 1100–1107. doi: https://doi.org/10.1084/jem.155.4.1100
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