Prolonged remissions were induced in mice bearing advanced BCL1 tumors by the combined approach of nonspecific cytoreductive therapy and administration of a tumor-reactive immunotoxin. Thus, the vast majority of the tumor cells (approximately 95%) were first killed by nonspecific cytoreductive therapy using total lymphoid irradiation (TLI) and splenectomy. The residual tumor cells were then eliminated by intravenous administration of an anti-delta immunotoxin. In three of four experiments, all animals treated in the above fashion appeared tumor free 12-16 wk later. In one experiment, blood cells from the mice in remission were transferred to normal BALB/c recipients, and the latter animals have not developed detectable tumor for the 6 mo of observation. Because 1-10 adoptively transferred BCL1 cells will cause tumor in normal BALB/c mice by 12 wk, the inability to transfer tumor to recipients might indicate that the donor animals were tumor free. In the remainder of the animals treated with the tumor-reactive immunotoxin there was a substantial remission in all animals, but the disease eventually reappeared. In contrast, all mice treated with the control immunotoxin or antibody alone relapsed significantly earlier (3-4 wk after splenectomy).
Article| June 01 1982
In vivo therapy of a murine B cell tumor (BCL1) using antibody-ricin A chain immunotoxins.
K A Krolick
J W Uhr
E S Vitetta
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1982) 155 (6): 1797–1809.
K A Krolick, J W Uhr, S Slavin, E S Vitetta; In vivo therapy of a murine B cell tumor (BCL1) using antibody-ricin A chain immunotoxins.. J Exp Med 1 June 1982; 155 (6): 1797–1809. doi: https://doi.org/10.1084/jem.155.6.1797
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