Expression of VH-coded mRNA molecules in T cells, antigen-specific T cell lines, or T cell hybridomas was not detected using four different VH DNA probes under conditions that permitted cross-hybridization between distantly related VH genes. In contrast, VH gene expression was readily detected in two B cell lymphomas and in splenic B cells. Less than one molecule per cell of RNA, exactly complementary to the DNA probes used, would have been detected in these T cell populations. The results thus seriously question the proposition that T cells use the B cell VH repertoire to code for antigen receptors.
A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells.
D J Kemp, J M Adams, P L Mottram, W R Thomas, I D Walker, J F Miller; A search for messenger RNA molecules bearing immunoglobulin VH nucleotide sequences in T cells.. J Exp Med 1 December 1982; 156 (6): 1848–1853. doi: https://doi.org/10.1084/jem.156.6.1848
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