Functional NK activity can be removed from human PBL and from phagocyte- and T cell-depleted LGL preparations by treatment with antisera specific for C-reactive protein (CRP) in the presence of complement (C). Pretreatment of NK effector cells with high concentrations of anti-CRP in the absence of C also depletes functional activity. These results indicate that CRP or an antigenically similar molecule is present on a population of NK effector cells. Fluorescent antibody studies in which biotin-avidin amplification was used confirm the presence of surface CRP (S-CRP) on a small percentage of nonphagocytic peripheral blood mononuclear cells. S-CRP readily caps off, which suggest that removal by capping obviates killing by this cell population. This indicates that S-CRP or a molecule that co-caps with S-CRP may be required for successful effector-target cell interaction. The addition of exogenous CRP or CRP-CPS complexes, however, does not alter NK responses. A subpopulation of lymphoid cells responsible for functional NK activity therefore appears to bear surface CRP.
Article| January 01 1983
Possible role for C-reactive protein in the human natural killer cell response.
R R Glaviano,
L L Baum
K K James
R R Glaviano
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1983) 157 (1): 301–311.
L L Baum, K K James, R R Glaviano, H Gewurz; Possible role for C-reactive protein in the human natural killer cell response.. J Exp Med 1 January 1983; 157 (1): 301–311. doi: https://doi.org/10.1084/jem.157.1.301
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