Studies of cellular events associated with antigen-induced triggering and differentiation of B cells would be greatly facilitated by the availability of homogeneous cell lines of antigen-specific lymphocytes that can be maintained in long-term culture. By combining the techniques of enrichment of lymphocytes for antigen-specific cells, cloning in soft agar, and long-term propagation of B cells we have been able to isolate, propagate, and maintain two lines of dinitrophenyl (DNP) -specific B lymphocytes. These cell lines are B lymphocytes that have 70% and greater than 80% DNP-specific rosette-forming cells, respectively. Both cell lines secrete small amounts of antibody spontaneously but can be stimulated by antigen in vitro in the presence of either supernatants from phytohemagglutinin-stimulated spleen cells or irradiated normal filler cells. Thus far these lines have been maintained in vitro for greater than 9 mo. They will be useful in studying factors associated with B cell response.
Article| January 01 1983
Long-term growth of lines of murine dinitrophenyl-specific B lymphocytes in vitro.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1983) 157 (1): 342–347.
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M Aldo-Benson, L Scheiderer; Long-term growth of lines of murine dinitrophenyl-specific B lymphocytes in vitro.. J Exp Med 1 January 1983; 157 (1): 342–347. doi: https://doi.org/10.1084/jem.157.1.342
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