Peyer's patch (PP) and mesenteric lymph node (MLN) cell cultures from young adult X-linked immunodeficient (xid) CBA/N and (CBA/N X DBA/2) F1 male mice support primary anti-sheep erythrocyte (SRBC) plaque-forming cell (PFC) responses, which suggests that gut-associated lymphoreticular tissue (GALT) contains a normal B lymphocyte subpopulation. Further support for this was provided by the observation that PP cells from xid mice gave responses to both TI-1 and TI-2 antigens that were similar to the responses of PP cell cultures from normal mice. Spleen cell cultures from xid mice were unresponsive to SRBC and TI-2 antigens. Proof that GALT of xid mice contain mature B lymphocytes was provided by the demonstration of PP B cells that bear a low density of surface immunoglobulin M. When these cells were separated by flow cytometry and immunized with trinitrophenyl (TNP)-Ficoll in vitro, good anti-TNP PFC responses were observed. These results suggest that GALT of young adult xid mice contain mature B cells and may represent the origin for the mature B cell responses seen in aged xid mice.
Article|
February 01 1983
Evidence for a mature B cell subpopulation in Peyer's patches of young adult xid mice.
J H Eldridge
H Kiyono
S M Michalek
J R McGhee
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1983) 157 (2): 789–794.
Citation
J H Eldridge, H Kiyono, S M Michalek, J R McGhee; Evidence for a mature B cell subpopulation in Peyer's patches of young adult xid mice.. J Exp Med 1 February 1983; 157 (2): 789–794. doi: https://doi.org/10.1084/jem.157.2.789
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