The bm 1 H-2Kb mutant differs from the parental strain C57BL/6 (B6) only at amino acid (AA) positions 152, 155, and 156 of the H-2K molecule. The H-2Ld molecule is structurally identical with the H-2 Kbm1 molecule from positions 146-162, thus including all three AA substitutions in Kbm1. In direct lysis and monolayer adsorption studies, B6 anti-bml cytotoxic T lymphocytes (CTL) were shown to include at least five distinct CTL subsets of the following specificities. (a) Uniquely reactive with Kbm1; (b) cross-reactive with Kk; (c) cross-reactive with Dk; (d) cross-reactive with H-2d minus Ld, and (e) cross-reactive with Ld. If B6 anti-bm1 CTL were directed against the primary AA-sequence difference, then all five subsets are expected to react with Ld. However, four out of five CTL subsets including a major population uniquely directed against Kbm1 failed to react with Ld. These findings strongly strengthen the notion that CTL recognize conformational determinants and not primary AA sequences.
Article|
November 01 1983
Direct demonstration that cytotoxic T lymphocytes recognize conformational determinants and not primary amino acid sequences.
L P De Waal
S G Nathenson
C J Melief
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1983) 158 (5): 1720–1726.
Citation
L P De Waal, S G Nathenson, C J Melief; Direct demonstration that cytotoxic T lymphocytes recognize conformational determinants and not primary amino acid sequences.. J Exp Med 1 November 1983; 158 (5): 1720–1726. doi: https://doi.org/10.1084/jem.158.5.1720
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