To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1-secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion.
Article| December 01 1984
Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s).
J E Layton
E S Vitetta
J W Uhr
P H Krammer
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1984) 160 (6): 1850–1863.
J E Layton, E S Vitetta, J W Uhr, P H Krammer; Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s).. J Exp Med 1 December 1984; 160 (6): 1850–1863. doi: https://doi.org/10.1084/jem.160.6.1850
Download citation file: