We have shown that cytotoxic T cell clones specific for the nominal antigen FL will bind high molecular weight (600,000 to 2,000,000) polyacrylamide and Ficoll polymers conjugated with 200-600 FL groups per molecule. Low molecular weight polymers (40,000) with the same epitope density did not give stable binding. A high molecular weight polymer with a lower epitope density also failed to bind. Taken together, these results suggest that a substantial degree of multivalence is a necessary factor in the stable binding of nominal antigen to T cell clones.
Antigen valence determines the binding of nominal antigen to cytolytic T cell clones.
R F Siliciano, R M Colello, A D Keegan, R Z Dintzis, H M Dintzis, H S Shin; Antigen valence determines the binding of nominal antigen to cytolytic T cell clones.. J Exp Med 1 August 1985; 162 (2): 768–773. doi: https://doi.org/10.1084/jem.162.2.768
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