Long-term cultured bone marrow cells were characterized with respect to a number of B and pre-B cell markers. Cells expressing ThB, B-220, and IgM were found within cultures set up according to the procedure of Whitlock and Witte. This culture system was modified by placing sorted pre-B cells (ThB+, IgM-) from bone marrow in culture with previously-established bone marrow adherent layers. These cultures commenced growth without the lag associated with the Whitlock cultures. These cultured nonadherent cells show a high frequency of IgM+ cells, but do not express either IgD or Ia, and we refer to them as immature B cells. Cells with a similar phenotype (IgM+, Ia-, IgD-) are found within the spleens of young but not adult mice. The phorbol ester PMA induces expression of IgD on the cultured immature B cells, but has no effect on Ia expression. This suggests that the processing of H chain RNA transcripts may be affected by protein kinase C. These results demonstrate that the appearance of IgM, IgD, and Ia are independently controlled in long-term cultured B-lineage cells.
Article|
April 01 1986
Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes.
J R Dasch
P P Jones
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1986) 163 (4): 938–951.
Citation
J R Dasch, P P Jones; Independent regulation of IgM, IgD, and Ia antigen expression in cultured immature B lymphocytes.. J Exp Med 1 April 1986; 163 (4): 938–951. doi: https://doi.org/10.1084/jem.163.4.938
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