We have shown previously that CD8+ T cells proliferate upon exposure to autologous, antigen primed CD4+ T cells, and suppress the response of fresh T cells to the priming antigen but not irrelevant antigens. The stimulus and target of suppression in this system appears to be the antigen receptor on the surface of CD4+ cells, rather than the nominal antigen. In the current study, alloantigen primed CD4+ inducer cells and IL-2-containing medium were used to generate clones of suppressor cells from several individuals. The clones inhibited the response of fresh autologous T cells only to the original allogeneic stimulator cell and to stimulator cells that shared HLA-DR antigens with the priming cell. The clones were also genetically restricted, since they inhibited the response of HLA-A,B-compatible but not HLA-A,B-incompatible individuals. The availability of a method for reproducibly generating antigen receptor-specific suppressor T cell clones in vitro should make it possible to clarify the mechanism, whereby such cells are activated and exert their suppressive effect.
Article|
September 01 1986
Generation of antigen receptor-specific suppressor T cell clones in man.
N Mohagheghpour
N K Damle
S Takada
E G Engleman
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1986) 164 (3): 950–955.
Citation
N Mohagheghpour, N K Damle, S Takada, E G Engleman; Generation of antigen receptor-specific suppressor T cell clones in man.. J Exp Med 1 September 1986; 164 (3): 950–955. doi: https://doi.org/10.1084/jem.164.3.950
Download citation file: