We have produced a T cell hybridoma line by fusion of an IL-2-dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line. The resultant hybridoma was rapidly growing, lymphokine independent, and expressed T3 in association with the TCR gamma/delta heterodimer. Several subclones of the hybridoma line produced easily detectable levels of IL-2 after stimulation by anti-T3 or Con A. The availability of these cloned cell lines should greatly facilitate further functional, biochemical, and molecular studies of the TCR delta chain.
Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer.
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W M Yokoyama, F Koning, G Stingl, J A Bluestone, J E Coligan, E M Shevach; Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer.. J Exp Med 1 June 1987; 165 (6): 1725–1730. doi: https://doi.org/10.1084/jem.165.6.1725
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