Primate bone marrow cells were infected with a retroviral vector carrying the genes for human adenosine deaminase (h-ADA) and bacterial neomycin resistance (neor). The infected cells were infused back into the lethally irradiated donor animals. Several monkeys fully reconstituted and were shown to express the h-ADA and neor genes at low levels in their recirculating hematopoietic cells for short periods of time.
Expression of human adenosine deaminase in nonhuman primates after retrovirus-mediated gene transfer.
P W Kantoff, A P Gillio, J R McLachlin, C Bordignon, M A Eglitis, N A Kernan, R C Moen, D B Kohn, S F Yu, E Karson; Expression of human adenosine deaminase in nonhuman primates after retrovirus-mediated gene transfer.. J Exp Med 1 July 1987; 166 (1): 219–234. doi: https://doi.org/10.1084/jem.166.1.219
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