A series of lambda+ murine hybridomas were derived from a BALB/c mouse after a single injection of anti-lambda 2 antibodies coupled to LPS. Nine lambda B cell clones (five lambda 2 and four lambda 3) were expected and seven reacted with antibodies specific for the C lambda 2 constant region but showed a particular isoelectric spectrum. Their RNA products did not hybridize with the V lambda probe. The partial DNA sequence of gene segments coding the unexpected light chain of one hybridoma shows that the V gene segment has only 55% homology with the V lambda 2 gene segment sequence and that J lambda 2 and probably C lambda 2 gene segments are used. Taken together, these results demonstrate the existence of a new lambda light chain.
Article| July 01 1987
A new variable region in mouse immunoglobulin lambda light chains.
P A Cazenave
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1987) 166 (1): 265–270.
P Sanchez, P A Cazenave; A new variable region in mouse immunoglobulin lambda light chains.. J Exp Med 1 July 1987; 166 (1): 265–270. doi: https://doi.org/10.1084/jem.166.1.265
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