The mouse MHC class I family includes genes encoded in four regions: H-2K, H-2D, Qa and Tla. While K/D genes are well characterized, relatively little is known about Qa or Tla genes. We have studied the transcription of a B10.P Qa region gene. DNA sequence comparisons of the transmembrane region, supported by Southern blot analysis of cosmid and genomic DNAs from BALB/c and C57BL/10, demonstrate the lambda 3a gene corresponds to Q4p. In both Northern blots and RNA protection experiments using probes derived from the 3' noncoding region, we found that Q4, like the H-2K and H-2D genes, is widely transcribed in B10.P tissues. These data demonstrate for the first time widespread transcription of a Qa gene.
Article| July 01 1987
Widespread transcription of a Qa region gene in adult mice.
M J Palmer
J A Frelinger
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1987) 166 (1): 95–108.
M J Palmer, J A Frelinger; Widespread transcription of a Qa region gene in adult mice.. J Exp Med 1 July 1987; 166 (1): 95–108. doi: https://doi.org/10.1084/jem.166.1.95
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