We have isolated the cDNA for human lymphocyte function-associated antigen 3 (LFA-3), the ligand of the T lymphocyte CD2 molecule. The identity of the clones was established by comparison of the deduced amino acid sequence to the LFA-3 NH2-terminal and tryptic peptide sequences. The cDNA defines a mature protein of 222 amino acids that structurally resembles typical membrane-anchored proteins. An extracellular domain with six N-linked glycosylation sites is followed by a hydrophobic putative transmembrane region and a short cytoplasmic domain. The mature glycoprotein is estimated to be 44-68% carbohydrate. Southern blots of human genomic DNA indicate that only one gene codes for human LFA-3. Northern blot analysis demonstrates that the LFA-3 mRNA of 1.3 kb is widely distributed in human tissues and cell lines.
Article|
October 01 1987
Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein.
B P Wallner,
B P Wallner
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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A Z Frey,
A Z Frey
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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R Tizard,
R Tizard
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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R J Mattaliano,
R J Mattaliano
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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C Hession,
C Hession
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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M E Sanders,
M E Sanders
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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M L Dustin,
M L Dustin
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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T A Springer
T A Springer
Biogen Research Corporation, Cambridge, Massachusetts 02142.
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B P Wallner
Biogen Research Corporation, Cambridge, Massachusetts 02142.
A Z Frey
Biogen Research Corporation, Cambridge, Massachusetts 02142.
R Tizard
Biogen Research Corporation, Cambridge, Massachusetts 02142.
R J Mattaliano
Biogen Research Corporation, Cambridge, Massachusetts 02142.
C Hession
Biogen Research Corporation, Cambridge, Massachusetts 02142.
M E Sanders
Biogen Research Corporation, Cambridge, Massachusetts 02142.
M L Dustin
Biogen Research Corporation, Cambridge, Massachusetts 02142.
T A Springer
Biogen Research Corporation, Cambridge, Massachusetts 02142.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1987) 166 (4): 923–932.
Citation
B P Wallner, A Z Frey, R Tizard, R J Mattaliano, C Hession, M E Sanders, M L Dustin, T A Springer; Primary structure of lymphocyte function-associated antigen 3 (LFA-3). The ligand of the T lymphocyte CD2 glycoprotein.. J Exp Med 1 October 1987; 166 (4): 923–932. doi: https://doi.org/10.1084/jem.166.4.923
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