A cell line, HAFTL-1, derived by in vitro transformation of fetal liver cells with v-Ha-ras, was found to have molecular and phenotypic characteristics of pro-B cells recently committed to the Ly-1+ B cell differentiation pathway. Stimulation of these cells with LPS resulted in their differentiation within either the B or myelomonocytic lineages. Thus, lines derived from LPS-stimulated HAFTL-1 cells were shown to be clonally related, as evidenced by common v-ras integrations, but to exhibit characteristics of pre-B cells (ThB expression, continuing DJ heavy chain rearrangements) or mature macrophages (expression of Mac-1 and Mac-2, lysozyme and nonspecific esterase production, phagocytosis) while maintaining their Ly-1+ phenotype. These results suggest that events resulting in the irrevocable commitment to a single lineage occur late in differentiation, at least within the pathway yielding Ly-1+ B cells and a proposed subpopulation of Ly-1+ monocytes and macrophages. Final commitment to these lineages is carefully orchestrated, as evidenced by restricted expression of Ly-5 isoforms and production of IgH transcripts.
Article|
July 01 1988
Relationships between B cell and myeloid differentiation. Studies with a B lymphocyte progenitor line, HAFTL-1.
W F Davidson,
W F Davidson
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
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J H Pierce,
J H Pierce
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
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S Rudikoff,
S Rudikoff
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
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H C Morse, 3rd
H C Morse, 3rd
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
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W F Davidson
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
J H Pierce
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
S Rudikoff
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
H C Morse, 3rd
Laboratory of Genetics, National Cancer Institute, Bethesda, Maryland 20892.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1988) 168 (1): 389–407.
Citation
W F Davidson, J H Pierce, S Rudikoff, H C Morse; Relationships between B cell and myeloid differentiation. Studies with a B lymphocyte progenitor line, HAFTL-1.. J Exp Med 1 July 1988; 168 (1): 389–407. doi: https://doi.org/10.1084/jem.168.1.389
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