Vaccinia infection interferes with the presentation of influenza Haemagglutinin (HA) and Nucleoprotein (NP) to class I-restricted CTL. The inhibitory effect is selective for certain epitopes, and is more profound during the late phase of infection. For influenza A/NT/60/68 NP, the block is present during both early and late phases of infection, and is selective for the COOH-terminal epitope defined by peptide 366-379, having no detectable effect on the presentation of the NH2-terminal epitope 50-63. The presentation of HA is inhibited only during the late phase of vaccinia infection. For both proteins, presentation is partially (NP) or completely (HA) restored by expression of rapidly degraded protein fragments in the vaccinia infected target cell. For HA, deletion of the NH2-terminal signal sequence completely overcomes the block. For NP, either a large NH2-terminal deletion or the construction of a rapidly degraded ubiquitin-NP fusion protein partially restores presentation. These results illustrate the relationship between degradation of viral proteins in the cytoplasm of an infected cell and recognition of epitopes at the cell surface by class I-restricted T cells.
Article|
October 01 1988
Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen.
A Townsend,
A Townsend
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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J Bastin,
J Bastin
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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K Gould,
K Gould
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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G Brownlee,
G Brownlee
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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M Andrew,
M Andrew
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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B Coupar,
B Coupar
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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D Boyle,
D Boyle
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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S Chan,
S Chan
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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G Smith
G Smith
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
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A Townsend
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
J Bastin
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
K Gould
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
G Brownlee
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
M Andrew
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
B Coupar
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
D Boyle
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
S Chan
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
G Smith
Institute for Molecular Medicine, John Radcliffe Hospital, Headington, Oxford.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1988) 168 (4): 1211–1224.
Citation
A Townsend, J Bastin, K Gould, G Brownlee, M Andrew, B Coupar, D Boyle, S Chan, G Smith; Defective presentation to class I-restricted cytotoxic T lymphocytes in vaccinia-infected cells is overcome by enhanced degradation of antigen.. J Exp Med 1 October 1988; 168 (4): 1211–1224. doi: https://doi.org/10.1084/jem.168.4.1211
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