We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.
Article|
November 01 1988
A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I.
D L Rosenstreich,
D L Rosenstreich
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
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J H Tu,
J H Tu
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
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P R Kinkade,
P R Kinkade
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
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I Maurer-Fogy,
I Maurer-Fogy
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
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J Kahn,
J Kahn
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
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R W Barton,
R W Barton
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
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P R Farina
P R Farina
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
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D L Rosenstreich
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
J H Tu
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
P R Kinkade
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
I Maurer-Fogy
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
J Kahn
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
R W Barton
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
P R Farina
Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1988) 168 (5): 1767–1779.
Citation
D L Rosenstreich, J H Tu, P R Kinkade, I Maurer-Fogy, J Kahn, R W Barton, P R Farina; A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I.. J Exp Med 1 November 1988; 168 (5): 1767–1779. doi: https://doi.org/10.1084/jem.168.5.1767
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