In the human there are three isotypic forms of MHC class II gene products (HLA-DR, -DQ, and -DP). The isotype-matched alpha-beta dimers are predominant but isotype-mismatched dimers can also be expressed (DR alpha-DQ beta). Here it is shown that the expression of the DR alpha-DQ beta dimer can be correlated to a high ratio of DR alpha/DR beta mRNA. The DR alpha chain expression was modulated by transfection of a sense and antisense DR alpha cDNA. Overexpression of DR alpha promoted the appearance of the DR alpha-DQ beta dimer. On the other hand, pre-existing DR alpha-DQ beta dimer expression was suppressed after antisense DR alpha cDNA transfection. Therefore, imbalanced expression of the alpha and beta chain from a given isotype could lead to the modification of HLA class II phenotype.
Modulation of HLA class II antigen expression by transfection of sense and antisense DR alpha cDNA.
V Lotteau, J Sands, L Teyton, P Turmel, D Charron, J L Strominger; Modulation of HLA class II antigen expression by transfection of sense and antisense DR alpha cDNA.. J Exp Med 1 January 1989; 169 (1): 351–356. doi: https://doi.org/10.1084/jem.169.1.351
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