We have generated an mAb, RR3-15, that recognizes murine TCRs containing the V beta 11 domain. Using this antibody to stain peripheral T cells, we have demonstrated that V beta 11-bearing T cells are largely absent from strains of mice that express the class II MHC molecule, I-E. Studies with F1 mice demonstrate that this effect is dominant, consistent with tolerance. The clonal deletion of V beta 11-bearing T cells appears to occur intrathymically, as immature but not mature V beta 11+ T cells are present in the thymus of I-E-bearing mice. Examination of B6 x DBA/2 recombinant inbred strains demonstrates that the expression of I-E molecules is necessary for the clonal deletion of V beta 11-bearing T cells, but that other non-MHC genes control the clonal deletion process, as well. Paradoxically, only a small fraction of V beta 11+ T cell hybridomas are I-E reactive.
The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells.
J Bill, O Kanagawa, D L Woodland, E Palmer; The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells.. J Exp Med 1 April 1989; 169 (4): 1405–1419. doi: https://doi.org/10.1084/jem.169.4.1405
Download citation file: