We show here an effective and novel approach to engineer peptide-based vaccines using a chemically defined system, known as multiple peptide antigen systems (MAPs), to protect an inbred mouse strain from infection against rodent malaria. 10 mono- and di-epitope MAP models containing different arrangements and stoichiometry of functional B and/or T helper cell epitopes from the circumsporozoite protein of Plasmodium berghei were used to immunize A/J mice. While these mice did not respond to the mono-epitope MAP bearing only the B or T epitope, very high titers of antibody and protective immunity against sporozoite challenge were elicited by di-epitope MAPs, particularly those with the B and T epitopes in tandem and present in equimolar amounts. These results, obtained in a well-defined rodent malaria model, indicate that MAPs may overcome some of the difficulties in the development of synthetic vaccines, not only for malaria but also for other infectious diseases.
Article|
January 01 1990
Incorporation of T and B epitopes of the circumsporozoite protein in a chemically defined synthetic vaccine against malaria.
J P Tam,
J P Tam
Rockefeller University, New York, New York 10021.
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P Clavijo,
P Clavijo
Rockefeller University, New York, New York 10021.
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Y A Lu,
Y A Lu
Rockefeller University, New York, New York 10021.
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V Nussenzweig,
V Nussenzweig
Rockefeller University, New York, New York 10021.
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R Nussenzweig,
R Nussenzweig
Rockefeller University, New York, New York 10021.
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F Zavala
F Zavala
Rockefeller University, New York, New York 10021.
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J P Tam
Rockefeller University, New York, New York 10021.
P Clavijo
Rockefeller University, New York, New York 10021.
Y A Lu
Rockefeller University, New York, New York 10021.
V Nussenzweig
Rockefeller University, New York, New York 10021.
R Nussenzweig
Rockefeller University, New York, New York 10021.
F Zavala
Rockefeller University, New York, New York 10021.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 171 (1): 299–306.
Citation
J P Tam, P Clavijo, Y A Lu, V Nussenzweig, R Nussenzweig, F Zavala; Incorporation of T and B epitopes of the circumsporozoite protein in a chemically defined synthetic vaccine against malaria.. J Exp Med 1 January 1990; 171 (1): 299–306. doi: https://doi.org/10.1084/jem.171.1.299
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