The mechanism of eosinophil secretion was studied in guinea pig eosinophils by measuring release of hexosaminidase from cell suspensions (greater than 98% pure) permeabilized with streptolysin-O and by whole-cell patch-clamp capacitance measurements. It is shown that release of eosinophil granule components occurs by an exocytotic mechanism in which individual granules fuse with the plasma membrane. Exocytosis can be induced by intracellular application of the nonhydrolyzable GTP analog guanosine-5'-O-(3-thiotriphosphate) (GTP-gamma-S), suggesting the involvement of a GTP-binding protein. The activation is modulated by the intracellular calcium concentration, with activation by GTP-gamma-S inducing transient elevations in the concentration of Ca2+. Thus, the nature and regulation of the release mechanism appear to be very similar to that of the mast cell and neutrophil.
Article|
March 01 1990
Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils.
O Nüsse,
O Nüsse
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
Search for other works by this author on:
M Lindau,
M Lindau
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
Search for other works by this author on:
O Cromwell,
O Cromwell
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
Search for other works by this author on:
A B Kay,
A B Kay
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
Search for other works by this author on:
B D Gomperts
B D Gomperts
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
Search for other works by this author on:
O Nüsse
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
M Lindau
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
O Cromwell
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
A B Kay
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
B D Gomperts
Biophysics Group, Freie Universität Berlin, Federal Republic of Germany.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 171 (3): 775–786.
Citation
O Nüsse, M Lindau, O Cromwell, A B Kay, B D Gomperts; Intracellular application of guanosine-5'-O-(3-thiotriphosphate) induces exocytotic granule fusion in guinea pig eosinophils.. J Exp Med 1 March 1990; 171 (3): 775–786. doi: https://doi.org/10.1084/jem.171.3.775
Download citation file: