T cell responses to the product of the minor lymphocyte stimulatory locus Mls-1a involve the selective use of TCR V beta domains (especially V beta 6 and V beta 8.1) and are generally considered to be restricted to the CD4+ mature subset. We show here that CD8+ (presumably MHC class I-restricted) T cells bearing V beta 6 or V beta 8.1 also respond preferentially to Mls-1a determinants either in vitro (in mixed leukocyte cultures) or in vivo (in an adoptive transfer system). In vitro responses of both CD4+ V beta 6+ and CD8+ V beta 6+ cells to Mls-1a were dependent upon the MHC haplotype of the stimulator cells, with I-E+ (H-2d or H-2k) alleles being much more stimulatory than I-E- (H-2q). These data strengthen the analogy between Mls gene products and other MHC class II-dependent superantigens such as the bacterial enterotoxins.
Article|
April 01 1990
CD8+ T cells respond clonally to Mls-1a-encoded determinants.
H R MacDonald,
H R MacDonald
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
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R K Lees,
R K Lees
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
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Y Chvatchko
Y Chvatchko
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
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H R MacDonald
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
R K Lees
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
Y Chvatchko
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 171 (4): 1381–1386.
Citation
H R MacDonald, R K Lees, Y Chvatchko; CD8+ T cells respond clonally to Mls-1a-encoded determinants.. J Exp Med 1 April 1990; 171 (4): 1381–1386. doi: https://doi.org/10.1084/jem.171.4.1381
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