Melanoma growth-stimulatory activity (MGSA), a peptide reported to be mitogenic for Hs294T human melanoma cells, has extensive sequence similarity to the neutrophil-activating peptide NAP-1/IL-8, suggesting functional similarities. To test this hypothesis, MGSA was chemically synthesized and tested for its effects on human neutrophils. It was found to induce chemotaxis, exocytosis of elastase, and changes in cytosolic-free calcium to an extent and at concentrations similar to NAP-1/IL-8. However, MGSA was considerably less potent than NAP-1/IL-8 in inducing the respiratory burst. Intradermal injections in rats of MGSA resulted in a massive accumulation of neutrophils. Our data demonstrate that, apart from its growth-stimulatory activity, MGSA is a potent inflammatory agonist with neutrophil-stimulating properties.
Article|
May 01 1990
Neutrophil-activating properties of the melanoma growth-stimulatory activity.
B Moser,
B Moser
Theodor-Kocher Institute, University of Bern, Switzerland.
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I Clark-Lewis,
I Clark-Lewis
Theodor-Kocher Institute, University of Bern, Switzerland.
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R Zwahlen,
R Zwahlen
Theodor-Kocher Institute, University of Bern, Switzerland.
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M Baggiolini
M Baggiolini
Theodor-Kocher Institute, University of Bern, Switzerland.
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B Moser
Theodor-Kocher Institute, University of Bern, Switzerland.
I Clark-Lewis
Theodor-Kocher Institute, University of Bern, Switzerland.
R Zwahlen
Theodor-Kocher Institute, University of Bern, Switzerland.
M Baggiolini
Theodor-Kocher Institute, University of Bern, Switzerland.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 171 (5): 1797–1802.
Citation
B Moser, I Clark-Lewis, R Zwahlen, M Baggiolini; Neutrophil-activating properties of the melanoma growth-stimulatory activity.. J Exp Med 1 May 1990; 171 (5): 1797–1802. doi: https://doi.org/10.1084/jem.171.5.1797
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