The Eta-1 gene specifies a secreted product of activated T cells and is associated with genetic resistance to infection by an obligate intracellular bacterium. Previous studies have suggested that eta-1 might affect the ability of macrophages to migrate to the site of bacterial infection and/or to inhibit intracellular bacterial growth. We therefore examined the interaction of eta-1 with macrophages in vitro and in vivo. We find that macrophages express approximately 10(4) eta-1 receptors/cell and each receptor has a Kd of approximately 5 x 10(-10) M. The subsequence of eta-1 containing an RGD motif is required for binding because a synthetic peptide containing the eta-1 RGD domain inhibited protein attachment to macrophages. We also found that subcutaneous inoculation of mice with eta-1 resulted in a cellular infiltrate comprised primarily of macrophages. We propose that the interaction between eta-1 and its receptor on macrophages results in a change in macrophage physiology resulting in accumulation of these cells at extravascular sites.
Article|
June 01 1990
Definition of a specific interaction between the early T lymphocyte activation 1 (Eta-1) protein and murine macrophages in vitro and its effect upon macrophages in vivo.
R P Singh,
R P Singh
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Search for other works by this author on:
R Patarca,
R Patarca
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Search for other works by this author on:
J Schwartz,
J Schwartz
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Search for other works by this author on:
P Singh,
P Singh
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Search for other works by this author on:
H Cantor
H Cantor
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Search for other works by this author on:
R P Singh
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
R Patarca
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
J Schwartz
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
P Singh
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
H Cantor
Dana Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 171 (6): 1931–1942.
Citation
R P Singh, R Patarca, J Schwartz, P Singh, H Cantor; Definition of a specific interaction between the early T lymphocyte activation 1 (Eta-1) protein and murine macrophages in vitro and its effect upon macrophages in vivo.. J Exp Med 1 June 1990; 171 (6): 1931–1942. doi: https://doi.org/10.1084/jem.171.6.1931
Download citation file: