Interleukin 5 (IL-5) acts on eosinophil differentiation and activation, suggesting the existence of a membrane receptor for IL-5 on eosinophils. Here, we report that 125I-labeled recombinant human IL-5 bound, at 4 degrees C, to high affinity receptors on human eosinophils. The association constant was higher for hypodense eosinophils (1.93 x 10(9) M-1) than for normodense cells (0.39 x 10(9) M-1), with a closely related number of receptor sites per cell. No specific binding occurred on neutrophils. The specific binding of IL-5 was induced by overnight incubation at 37 degrees C of human eosinophils with granulocyte/macrophage (GM)-CSF. The levels of increase were significantly higher for normodense than for hypodense eosinophils, suggesting a previous in vivo activation of the later subpopulation by GM-CSF. IL-3 was ineffective by itself but synergistically enhanced the effect of GM-CSF. Specificity studies showed that the binding of 125I-labeled IL-5 was inhibited by IL-5, but not by other cytokines, on human eosinophils. These results show the existence of a specific binding site for IL-5 on human eosinophils with a variable affinity on eosinophil hypodense or normodense subpopulations, as previously reported for other membrane receptors.
Article|
November 01 1990
Characterization of a receptor for interleukin 5 on human eosinophils: variable expression and induction by granulocyte/macrophage colony-stimulating factor.
J Chihara,
J Chihara
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
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J Plumas,
J Plumas
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
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V Gruart,
V Gruart
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
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J Tavernier,
J Tavernier
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
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L Prin,
L Prin
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
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A Capron,
A Capron
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
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M Capron
M Capron
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
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J Chihara
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
J Plumas
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
V Gruart
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
J Tavernier
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
L Prin
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
A Capron
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
M Capron
Centre d'Immunologie et de Biologie Parasitaire, Unité Mixte INSERM U167, CNRS 624, Institut Pasteur, Lille, France.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1990) 172 (5): 1347–1351.
Citation
J Chihara, J Plumas, V Gruart, J Tavernier, L Prin, A Capron, M Capron; Characterization of a receptor for interleukin 5 on human eosinophils: variable expression and induction by granulocyte/macrophage colony-stimulating factor.. J Exp Med 1 November 1990; 172 (5): 1347–1351. doi: https://doi.org/10.1084/jem.172.5.1347
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