Much of T and B lymphocyte receptor diversity derives from the addition of nontemplated N regions at the junctions of receptor gene elements, although fetal T cells expressing gamma/delta receptors lack N regions. I have sequenced immunoglobulin H chain variable regions of PCR-amplified DNA and cDNA from fetal and newborn mouse liver and spleen cells. These sequences showed an absence of N regions. Only 1/87 DNA sequences and 17/146 RNA sequences contained N regions, in striking contrast to adult Ig sequences. These data show that N region insertion is a developmentally regulated process in B cells as well as in T cells, and demonstrate that receptor diversity in neonatal B cells is limited by the absence of N regions as well as by biased usage of Vh genes.
Article| November 01 1990
Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences.
A J Feeney
Division of Immunology, Medical Biology Institute, La Jolla, California 92037.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1990) 172 (5): 1377–1390.
- Views Icon Views
- Share Icon Share
- Search Site
A J Feeney; Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences.. J Exp Med 1 November 1990; 172 (5): 1377–1390. doi: https://doi.org/10.1084/jem.172.5.1377
Download citation file: