P21ras proteins are thought to play an important role in cell proliferation and differentiation. Single nucleotide mutations in the encoding cellular proto-oncogenes often result in p21ras proteins with transforming activity. Such activated ras oncogenes have been demonstrated in a variety of human malignancies and also in preneoplastic changes. Using a synthetic peptide corresponding to amino acids 5-16 of mutated p21ras proteins with an exchange of the normal glycine at position 12 by valine, it is shown here that human CD4+ T cells specifically recognize the mutated protein sequence and can be generated as antigen-specific T lymphocyte lines. The fact that these T lines did not crossreact to the sequence of normal p21ras proteins offers new perspectives for specific immunotherapy of human malignancies and even precancerous lesions.
Article|
January 01 1991
Human T lymphocytes recognize a peptide of single point-mutated, oncogenic ras proteins.
S Jung,
S Jung
Department of Neurology, University of Würzburg, Federal Republic of Germany.
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H J Schluesener
H J Schluesener
Department of Neurology, University of Würzburg, Federal Republic of Germany.
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S Jung
Department of Neurology, University of Würzburg, Federal Republic of Germany.
H J Schluesener
Department of Neurology, University of Würzburg, Federal Republic of Germany.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 173 (1): 273–276.
Citation
S Jung, H J Schluesener; Human T lymphocytes recognize a peptide of single point-mutated, oncogenic ras proteins.. J Exp Med 1 January 1991; 173 (1): 273–276. doi: https://doi.org/10.1084/jem.173.1.273
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