We have resolved B220+ IgM- B-lineage cells in mouse bone marrow into four fractions based on differential cell surface expression of determinants recognized by S7 (leukosialin, CD43), BP-1, and 30F1 (heat stable antigen). Functional differences among these fractions can be correlated with Ig gene rearrangement status. The largest fraction, lacking S7, consists of pre-B cells whereas the others, expressing S7, include B lineage cells before pre-B. These S7+ fractions, provisionally termed Fr. A, Fr. B, and Fr. C, can differentiate in a stromal layer culture system. Phenotypic alteration during such culture suggests an ordering of these stages from Fr. A to Fr. B to Fr. C and thence to S7- pre-B cells. Using polymerase chain reaction amplification with pairs of oligonucleotide primers for regions 5' of JH1, DFL16.1, and Jk1, we find that the Ig genes of Fr. A are in germline configuration, whereas Fr. B and C are pro-B cell stages with increasing D-J rearrangement, but no V-D-J. Finally, functional analysis demonstrates that the proliferative response to IL-7, an early B lineage growth factor, is restricted to S7+ stages and, furthermore, that an additional, cell contact-mediated signal is essential for survival of Fr. A.
Article|
May 01 1991
Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow.
R R Hardy,
R R Hardy
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
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C E Carmack,
C E Carmack
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
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S A Shinton,
S A Shinton
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
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J D Kemp,
J D Kemp
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
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K Hayakawa
K Hayakawa
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
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R R Hardy
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
C E Carmack
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
S A Shinton
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
J D Kemp
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
K Hayakawa
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 173 (5): 1213–1225.
Citation
R R Hardy, C E Carmack, S A Shinton, J D Kemp, K Hayakawa; Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow.. J Exp Med 1 May 1991; 173 (5): 1213–1225. doi: https://doi.org/10.1084/jem.173.5.1213
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