Presentation of antigen in the form of immune complexes to B lymphocytes by follicular dendritic cells (FDC) is considered to be a central step in the generation of memory B cells. During this process, which takes place in the microenvironment of the germinal center, B cells and FDC are in close physical contact. In the present study, we have explored the molecular basis of FDC-B cell interaction by using FDC and B cells derived from human tonsils. We found that FDC express high levels of the adhesion receptors intercellular adhesion molecule 1 (ICAM-1 [CD54]) and vascular cell adhesion molecule 1 (VCAM-1), while the B lymphocytes express lymphocyte function-associated antigen 1 (LFA-1 [CD11a/18]), very late antigen 4 (VLA-4 [CD49d], and CD44. Furthermore, we established that both the LFA-1/ICAM-1 and VLA-4/VCAM-1 adhesion pathways are involved in FDC-B lymphocyte binding, and therefore, these pathways might be essential in affinity selection of B cells and in the formation of B memory cells.
Article|
June 01 1991
Adhesion of human B cells to follicular dendritic cells involves both the lymphocyte function-associated antigen 1/intercellular adhesion molecule 1 and very late antigen 4/vascular cell adhesion molecule 1 pathways.
G Koopman,
G Koopman
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
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H K Parmentier,
H K Parmentier
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
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H J Schuurman,
H J Schuurman
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
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W Newman,
W Newman
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
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C J Meijer,
C J Meijer
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
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S T Pals
S T Pals
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
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G Koopman
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
H K Parmentier
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
H J Schuurman
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
W Newman
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
C J Meijer
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
S T Pals
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 173 (6): 1297–1304.
Citation
G Koopman, H K Parmentier, H J Schuurman, W Newman, C J Meijer, S T Pals; Adhesion of human B cells to follicular dendritic cells involves both the lymphocyte function-associated antigen 1/intercellular adhesion molecule 1 and very late antigen 4/vascular cell adhesion molecule 1 pathways.. J Exp Med 1 June 1991; 173 (6): 1297–1304. doi: https://doi.org/10.1084/jem.173.6.1297
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