A T cell line termed DIL2 has been derived from an infant with a polyclonal T cell receptor (TCR)/CD3 cell surface expression defect. Indirect immunofluorescence showed that the expression of certain TCR/CD3 epitopes (like those detected by WT31 and BMA031 monoclonals) was strongly reduced (around five-fold) on DIL2, whereas other epitopes (like those detected by SP34 and Leu4) were only around two-fold lower than in normal T cell lines. Specific immunoprecipitates of surface-radioiodinated DIL2 cells contained TCR-alpha, TCR-beta, CD3-delta, CD3-epsilon and TCR-zeta chains, but lacked CD3-gamma. This structural TCR/CD3 variant was, however, capable of transducing certain activation signals, since normal proliferation and a low but significant calcium flux was observed in DIL2 cells after engagement with specific antibodies. Our data suggest that a functional TCR/CD3 complex can be expressed on the surface of T cells in the absence of CD3-gamma.
Article|
August 01 1991
Expression and function of a variant T cell receptor complex lacking CD3-gamma.
P Pérez-Aciego,
P Pérez-Aciego
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
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B Alarcón,
B Alarcón
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
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A Arnaiz-Villena,
A Arnaiz-Villena
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
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C Terhorst,
C Terhorst
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
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M Timón,
M Timón
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
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O G Segurado,
O G Segurado
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
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J R Regueiro
J R Regueiro
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
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P Pérez-Aciego
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
B Alarcón
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
A Arnaiz-Villena
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
C Terhorst
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
M Timón
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
O G Segurado
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
J R Regueiro
Department of Immunology, Hospital 12 de Octubre, Madrid, Spain.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 174 (2): 319–326.
Citation
P Pérez-Aciego, B Alarcón, A Arnaiz-Villena, C Terhorst, M Timón, O G Segurado, J R Regueiro; Expression and function of a variant T cell receptor complex lacking CD3-gamma.. J Exp Med 1 August 1991; 174 (2): 319–326. doi: https://doi.org/10.1084/jem.174.2.319
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