Pfs25 is a sexual stage antigen of Plasmodium falciparum that is expressed on the surface of zygote and ookinete forms of the parasite. Monoclonal antibodies directed against native Pfs25 can block completely the development of P. falciparum oocysts in the midgut of the mosquito vector. Thus, this 25-kD protein is a potential vaccine candidate for eliciting transmission-blocking immunity in inhabitants of malaria endemic regions. We have synthesized, by secretion from yeast, a polypeptide analogue of Pfs25 that reacts with conformation-dependent monoclonal antibodies, and elicits transmission-blocking antibodies when used to immunize mice and monkeys in conjunction with a muramyl tripeptide adjuvant. Our results suggest the further evaluation of recombinant DNA-derived Pfs25 in transmission-blocking vaccination studies in humans.
Article|
November 01 1991
Recombinant Pfs25 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in experimental animals.
P J Barr,
P J Barr
Chiron Corporation, Emeryville, California 94608.
Search for other works by this author on:
K M Green,
K M Green
Chiron Corporation, Emeryville, California 94608.
Search for other works by this author on:
H L Gibson,
H L Gibson
Chiron Corporation, Emeryville, California 94608.
Search for other works by this author on:
I C Bathurst,
I C Bathurst
Chiron Corporation, Emeryville, California 94608.
Search for other works by this author on:
I A Quakyi,
I A Quakyi
Chiron Corporation, Emeryville, California 94608.
Search for other works by this author on:
D C Kaslow
D C Kaslow
Chiron Corporation, Emeryville, California 94608.
Search for other works by this author on:
P J Barr
Chiron Corporation, Emeryville, California 94608.
K M Green
Chiron Corporation, Emeryville, California 94608.
H L Gibson
Chiron Corporation, Emeryville, California 94608.
I C Bathurst
Chiron Corporation, Emeryville, California 94608.
I A Quakyi
Chiron Corporation, Emeryville, California 94608.
D C Kaslow
Chiron Corporation, Emeryville, California 94608.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1991) 174 (5): 1203–1208.
Citation
P J Barr, K M Green, H L Gibson, I C Bathurst, I A Quakyi, D C Kaslow; Recombinant Pfs25 protein of Plasmodium falciparum elicits malaria transmission-blocking immunity in experimental animals.. J Exp Med 1 November 1991; 174 (5): 1203–1208. doi: https://doi.org/10.1084/jem.174.5.1203
Download citation file: