CD3 zeta and CD3 eta form disulfide-linked homo- or heterodimers important in targeting partially assembled Ti alpha-beta/CD3 gamma delta epsilon T cell receptor (TCR) complexes to the cell surface and transducing stimulatory signals after antigen recognition. Here we identify a new TCR isoform expressed on splenic CD2+, CD3/Ti alpha-beta+, CD4-, CD8-, CD16+, NK1.1+ mouse large granular lymphocytes (LGL), which are devoid of CD3 zeta and CD3 eta proteins. The TCRs of this subset contain homodimers of the gamma subunit of the high affinity receptor for IgE (Fc epsilon RI gamma) in lieu of CD3 zeta and/or CD3 eta proteins. The LGL display natural killer-like activity and are cytotoxic for B cell hybridomas producing anti-CD3 epsilon and anti-CD16 monoclonal antibodies, demonstrating the signaling capacity of both TCR and CD16 in this cell type. These findings provide evidence for an additional level of complexity of TCR signal transduction isoforms in naturally occurring T cell subsets.
Article|
January 01 1992
T cell receptor complexes containing Fc epsilon RI gamma homodimers in lieu of CD3 zeta and CD3 eta components: a novel isoform expressed on large granular lymphocytes.
S Koyasu,
S Koyasu
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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L D'Adamio,
L D'Adamio
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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A R Arulanandam,
A R Arulanandam
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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S Abraham,
S Abraham
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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L K Clayton,
L K Clayton
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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E L Reinherz
E L Reinherz
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
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S Koyasu
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
L D'Adamio
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
A R Arulanandam
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
S Abraham
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
L K Clayton
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
E L Reinherz
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1992) 175 (1): 203–209.
Citation
S Koyasu, L D'Adamio, A R Arulanandam, S Abraham, L K Clayton, E L Reinherz; T cell receptor complexes containing Fc epsilon RI gamma homodimers in lieu of CD3 zeta and CD3 eta components: a novel isoform expressed on large granular lymphocytes.. J Exp Med 1 January 1992; 175 (1): 203–209. doi: https://doi.org/10.1084/jem.175.1.203
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