Humoral immune responses are initiated by binding of antigen to the immunoglobulins (Igs) on the plasma membrane of B lymphocytes. On the cell surface, Ig forms a complex with several other proteins, two of which, MB-1 and B29, have been implicated in receptor assembly. We have reconstituted Ig receptor function in T lymphocytes by transfection of cloned receptor components. We found that efficient transport of IgM to the surface of T cells required coexpression of B29. Furthermore, IgM and B29 alone were sufficient to reconstitute antigen-specific signal transduction by Ig in the transfected T cells. Crosslinking of IgM with either antireceptor antibodies or antigen induced a calcium flux, phosphoinositol turnover, and interleukin secretion in T cells. These experiments establish a requirement for B29 in Ig receptor function, and suggest that the signaling apparatus of T and B cells is structurally homologous.
Functional reconstitution of an immunoglobulin antigen receptor in T cells.
T E Costa, R R Franke, M Sanchez, Z Misulovin, M C Nussenzweig; Functional reconstitution of an immunoglobulin antigen receptor in T cells.. J Exp Med 1 June 1992; 175 (6): 1669–1676. doi: https://doi.org/10.1084/jem.175.6.1669
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