A common feature of T cell acute lymphoblastic leukemias (T-ALLs) is the presence of structural alteration of the 5' part of the tal-1 locus, localized on chromosomal band 1p32. These alterations consist of either a t(1;14)(p32;q11) chromosomal translocation (3% of T-ALLs) or tald submicroscopic deletion (12-25% additional T-ALLs). We have characterized a case of T-ALL with t(1;14)(p32;q11) in which, unlike the majority of t(1;14), the recombination with the T cell receptor delta elements affected the 3' side of the tal-1 locus. In this case, tal-1 transcription is initiated from a promoter located within the fourth exon similarly to the DU 528 cell line. In a T-ALL bearing a t(1;14) affecting the 5' part of tal-1, two types of tal-1 transcripts were observed, namely those probably initiated from the D delta region juxtaposed to tal-1 by the translocation, and those from the exon 4 promoter. It is interesting that this exon 4 promotion was also found in leukemic T cell lines and T-ALL samples without apparent tal-1 genomic alteration. In contrast, no transcript initiated from the exon 4 promoter was found in T-ALL with tald1 or tald2 deletion. In these cells, tal-1 is expressed via SIL-tal-1 fused transcripts. Finally, this exon 4 initiation was detected neither in normal bone marrow, nor in malignant cells from the erythroid/megakaryocytic lineages. Taken as a whole, these data suggest that the exon 4 promoter is specifically active in T cell lineage.
Article|
October 01 1992
A third tal-1 promoter is specifically used in human T cell leukemias.
O Bernard,
O Bernard
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
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O Azogui,
O Azogui
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
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N Lecointe,
N Lecointe
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
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F Mugneret,
F Mugneret
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
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R Berger,
R Berger
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
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C J Larsen,
C J Larsen
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
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D Mathieu-Mahul
D Mathieu-Mahul
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
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O Bernard
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
O Azogui
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
N Lecointe
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
F Mugneret
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
R Berger
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
C J Larsen
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
D Mathieu-Mahul
U. 301 Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Génétique Moléculaire (IGM), France.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1992) 176 (4): 919–925.
Citation
O Bernard, O Azogui, N Lecointe, F Mugneret, R Berger, C J Larsen, D Mathieu-Mahul; A third tal-1 promoter is specifically used in human T cell leukemias.. J Exp Med 1 October 1992; 176 (4): 919–925. doi: https://doi.org/10.1084/jem.176.4.919
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