T cell receptor V gamma genes rearrange to the J gamma 1 gene segment in a highly ordered fashion during development. We demonstrate a striking correlation between the pattern of expression of unrearranged V gamma genes and the timing of their rearrangement. Thus, the increases in V gamma 2 rearrangements, and decreases in V gamma 3 and V gamma 4 rearrangements observed during development are paralleled by increasing or decreasing levels of the corresponding unrearranged V gene transcript. We also provide evidence that both the V gamma 3 and V gamma 4 genes are accessible in mature V gamma 3+ cells, but that the V gamma 4 gene may be inaccessible in the progenitors of V gamma 3 cells. The results suggest that regulated local accessibility of the chromatin surrounding V gamma genes is responsible for ordered V gamma gene rearrangement during development.
Ordered rearrangement of variable region genes of the T cell receptor gamma locus correlates with transcription of the unrearranged genes.
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J P Goldman, D M Spencer, D H Raulet; Ordered rearrangement of variable region genes of the T cell receptor gamma locus correlates with transcription of the unrearranged genes.. J Exp Med 1 March 1993; 177 (3): 729–739. doi: https://doi.org/10.1084/jem.177.3.729
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