Natural killer (NK) cells kill normal and transformed hematopoietic cells that lack expression of major histocompatibility complex (MHC) class I antigens. Lysis of HLA-negative Epstein Barr virus-transformed B lymphoblastoid cell lines (B-LCL) by human NK cell clones can be inhibited by transfection of the target cells with certain HLA-A, -B, or -C alleles. NK cell clones established from an individual demonstrate clonal heterogeneity in HLA recognition and a single NK clone can recognize multiple alleles. We describe a potential human NK cell receptor (NKB1) for certain HLA-B alleles (e.g., HLA-B*5101 and-B*5801) identified by the mAb DX9. NKB1 is a 70-kD glycoprotein that is expressed on a subset of NK cells and NK cell clones. DX9 monoclonal antibody (mAb) specifically inhibits the interaction between NK cell clones and B-LCL targets transfected with certain HLA-B alleles, but does not affect recognition of HLA-A or HLA-C antigens. An individual NK cell clone can independently recognize B-LCL targets transfected with HLA-B or HLA-C antigens; however, DX9 mAb only affects interaction with transfectants expressing certain HLA-B alleles. These findings demonstrate the existence of NK cell receptors involved in the recognition of HLA-B and imply the presence of multiple receptors for MHC on an individual NK clone.
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August 01 1994
NKB1: a natural killer cell receptor involved in the recognition of polymorphic HLA-B molecules.
V Litwin,
V Litwin
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
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J Gumperz,
J Gumperz
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
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P Parham,
P Parham
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
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J H Phillips,
J H Phillips
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
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L L Lanier
L L Lanier
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
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V Litwin
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
J Gumperz
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
P Parham
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
J H Phillips
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
L L Lanier
Department of Human Immunology, DNAX Research Institute of Molecular and Cellular Biology, Inc., Palo Alto, California 94304.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1994) 180 (2): 537–543.
Citation
V Litwin, J Gumperz, P Parham, J H Phillips, L L Lanier; NKB1: a natural killer cell receptor involved in the recognition of polymorphic HLA-B molecules.. J Exp Med 1 August 1994; 180 (2): 537–543. doi: https://doi.org/10.1084/jem.180.2.537
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