Efficient activation and regulation of the cellular immune response requires engagement of T cell accessory molecules as well as the antigen-specific T cell receptor. The lymphocyte function-associated antigen (LFA) 3 (CD58)/CD2 accessory pathway, one of the first discovered, has been extensively characterized in terms of structure and function of the CD2 molecule, which is present on all T lymphocytes and natural killer cells of the human immune system. The binding site of human CD2 for LFA-3 has been localized to two epitopes on one face of the first immunoglobulin (Ig)-like domain of this two-domain, Ig superfamily molecule. Human LFA-3 is genetically linked and is 21% identical in amino acid sequence to CD2, suggesting that this adhesive pair may have evolved from a single ancestral molecule. We have aligned the amino acid sequences of LFA-3 and CD2 and mutagenized selected amino acids in the first domain of LFA-3 that are analogous to those implicated in the binding site of CD2. The data show that K30 and K34, in the predicted C-C' loop, and D84, in the predicted F-G loop of LFA-3, are involved in binding to CD2, suggesting that two complementary sites on one face of the first domain of each molecule bind to each other.
Article|
January 01 1995
Amino acid residues required for binding of lymphocyte function-associated antigen 3 (CD58) to its counter-receptor CD2.
L Osborn,
L Osborn
Biogen Inc, Cambridge, Massachusetts 02142.
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E S Day,
E S Day
Biogen Inc, Cambridge, Massachusetts 02142.
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G T Miller,
G T Miller
Biogen Inc, Cambridge, Massachusetts 02142.
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M Karpusas,
M Karpusas
Biogen Inc, Cambridge, Massachusetts 02142.
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R Tizard,
R Tizard
Biogen Inc, Cambridge, Massachusetts 02142.
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S C Meuer,
S C Meuer
Biogen Inc, Cambridge, Massachusetts 02142.
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P S Hochman
P S Hochman
Biogen Inc, Cambridge, Massachusetts 02142.
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L Osborn
Biogen Inc, Cambridge, Massachusetts 02142.
E S Day
Biogen Inc, Cambridge, Massachusetts 02142.
G T Miller
Biogen Inc, Cambridge, Massachusetts 02142.
M Karpusas
Biogen Inc, Cambridge, Massachusetts 02142.
R Tizard
Biogen Inc, Cambridge, Massachusetts 02142.
S C Meuer
Biogen Inc, Cambridge, Massachusetts 02142.
P S Hochman
Biogen Inc, Cambridge, Massachusetts 02142.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1995) 181 (1): 429–434.
Citation
L Osborn, E S Day, G T Miller, M Karpusas, R Tizard, S C Meuer, P S Hochman; Amino acid residues required for binding of lymphocyte function-associated antigen 3 (CD58) to its counter-receptor CD2.. J Exp Med 1 January 1995; 181 (1): 429–434. doi: https://doi.org/10.1084/jem.181.1.429
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