Previous studies from our laboratory demonstrated an inverse relationship between the expression level of inducible nitric oxide synthase (iNOS) and the metastatic potential of murine K-1735 melanoma cells. The purpose of this study was to provide direct evidence that the expression of iNOS suppresses metastatic potential of melanoma cells. Highly metastatic K-1735 clone 4 cells (C4.P), which express low levels of iNOS, were transfected with a functional iNOS (C4.L8), inactive-mutated iNOS (C4.S2), or neomycin-resistance (C4.Neo) genes in medium containing 3 mM NG-methyl-L-arginine (NMA). Positive transfectants were identified by Southern and Northern blot analyses and homogeneous staining with a specific anti-iNOS monoclonal antibody. Semiconfluent cultures of C4.P (parental), C4.Neo.3 (control transfection), C4.S2.3 (inactive iNOS), and C4.L8.5 (functional iNOS) were harvested, and viable cells were injected intravenously into syngeneic C3H/HeN mice and allogeneic BALB/c nude mice. C4.P, C4.Neo.3, and C4.S2.3 cells were highly metastatic whereas C4.L8.5 cells were not metastatic. Experiments with [125I]dUrd-labeled tumor cells demonstrated that the initial arrest in the lung microvasculature did not differ among the lines, but that C4.L8.5 cells died by 48-72 h after injection. Enhanced survival of all K-1735 C4 cells (including C4.L8.5) was found in mice given twice daily injections of 20 mg NMA. The C4.L8.5 cells produced slow growing subcutaneous tumors in nude mice, whereas the other three lines produced fast growing tumors. In vitro studies confirmed that in the absence of NMA the expression of iNOS in C4.L8.5 cells induced apoptosis. Collectively, these data demonstrate that the expression of recombinant iNOS in melanoma cells is associated with apoptosis, suppression of tumorigenicity, and abrogation of metastasis.
Article|
April 01 1995
Transfection with the inducible nitric oxide synthase gene suppresses tumorigenicity and abrogates metastasis by K-1735 murine melanoma cells.
K Xie,
K Xie
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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S Huang,
S Huang
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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Z Dong,
Z Dong
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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S H Juang,
S H Juang
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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M Gutman,
M Gutman
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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Q W Xie,
Q W Xie
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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C Nathan,
C Nathan
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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I J Fidler
I J Fidler
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
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K Xie
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
S Huang
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Z Dong
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
S H Juang
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
M Gutman
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Q W Xie
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
C Nathan
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
I J Fidler
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1995) 181 (4): 1333–1343.
Citation
K Xie, S Huang, Z Dong, S H Juang, M Gutman, Q W Xie, C Nathan, I J Fidler; Transfection with the inducible nitric oxide synthase gene suppresses tumorigenicity and abrogates metastasis by K-1735 murine melanoma cells.. J Exp Med 1 April 1995; 181 (4): 1333–1343. doi: https://doi.org/10.1084/jem.181.4.1333
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