Focal adhesion kinase (pp125FAK) is localized to focal adhesions and tyrosine phosphorylated by the engagement of beta 1 integrins. However, it is unclear how pp125FAK is linked to integrin molecules. We demonstrate that pp125FAK is directly associated with paxillin, a 68-kD cytoskeleton protein. The COOH-terminal domain of pp125FAK spanning FAK residues 919-1042 is sufficient for paxillin binding and has vinculin-homologous amino acids, which are essential for paxillin binding. Microinjection and subsequent immunohistochemical analysis reveal that glutathione S-transferase-FAK fusion proteins, which bind to paxillin, localize to focal adhesions, whereas fusion proteins with no paxillin-binding activity do not localize to focal adhesions. These findings strongly suggest that pp125FAK is localized to focal adhesions by the direct association with paxillin.
Article|
October 01 1995
Direct association of pp125FAK with paxillin, the focal adhesion-targeting mechanism of pp125FAK.
K Tachibana,
K Tachibana
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
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T Sato,
T Sato
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
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N D'Avirro,
N D'Avirro
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
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C Morimoto
C Morimoto
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
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K Tachibana
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
T Sato
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
N D'Avirro
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
C Morimoto
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.
Online Issn: 1540-9538
Print Issn: 0022-1007
J Exp Med (1995) 182 (4): 1089–1099.
Citation
K Tachibana, T Sato, N D'Avirro, C Morimoto; Direct association of pp125FAK with paxillin, the focal adhesion-targeting mechanism of pp125FAK.. J Exp Med 1 October 1995; 182 (4): 1089–1099. doi: https://doi.org/10.1084/jem.182.4.1089
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