Patients with primary biliary cirrhosis frequently develop autoantibodies directed to gp210, a major glycoprotein of the nuclear pore complex. This protein contains a large glycosylated cisternal domain, a single transmembrane segment, and a short cytoplasmic tail. It has been previously shown that autoantibodies from primary biliary cirrhosis patients exclusively react with the cytoplasmic tail. We demonstrate that autoantibodies against gp210 recognize at least two different epitopes. 4 out of 12 anti-gp210 positive sera reacted with the fragment consisting of the cytoplasmic tail, and 8 sera targeted a novel epitope located within the large glycosylated lumenal domain. Moreover, our data prove that carbohydrate moieties are an essential part of this novel epitope. We propose, therefore, that future screening assays should be performed with antigens possessing both epitopes to detect all sera with anti-gp210 specificity.
Article|
October 01 1995
Autoantibodies from patients with primary biliary cirrhosis preferentially react with the amino-terminal domain of nuclear pore complex glycoprotein gp210.
J Wesierska-Gadek,
J Wesierska-Gadek
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
Search for other works by this author on:
H Hohenauer,
H Hohenauer
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
Search for other works by this author on:
E Hitchman,
E Hitchman
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
Search for other works by this author on:
E Penner
E Penner
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
Search for other works by this author on:
J Wesierska-Gadek
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
H Hohenauer
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
E Hitchman
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
E Penner
Institute of Tumorbiology-Cancer Research, University of Vienna, Austria.
Online ISSN: 1540-9538
Print ISSN: 0022-1007
J Exp Med (1995) 182 (4): 1159–1162.
Citation
J Wesierska-Gadek, H Hohenauer, E Hitchman, E Penner; Autoantibodies from patients with primary biliary cirrhosis preferentially react with the amino-terminal domain of nuclear pore complex glycoprotein gp210.. J Exp Med 1 October 1995; 182 (4): 1159–1162. doi: https://doi.org/10.1084/jem.182.4.1159
Download citation file: